This type of diabetes is observed in approximately 45% of all pregnancies, due to placental hormones that. Raw camel milk properties on alloxaninduced diabetic. Antidiabetic effect of ipomoea batatas in normal and. We report herein the findings of this research work. Alloxaninduced diabetes causes morphological and ultrastructural. Thereafter, several authors have used alloxaninduced diabetes model as a study tool to elucidate the pathophysiology of the disease and much more as a search engine for antidiabetic compounds with better therapeutic characteristics. Treatment of alloxaninduced diabetic rats with metformin or.
It has been successfully induced in a variety of animal species. Szkudelski department of animal physiology and biochemistry, university of agriculture, poznan, poland received november 2, 2000 accepted march 20, 2001 summary alloxan and streptozotocin are widely used to induce experimental diabetes in animals. The responses are accompanied by corresponding inverse changes in plasma insulin and sequential ultrastructural. Hypoglycemic effects in alloxaninduced diabetic rats of the. Disruption of the tubular basement membrane led to. Biochemical blood glucose, serum insulin and glutathione peroxidase and histopathological analysis of pancreas was performed in all three experimental. Cinnamon has been widely recognized as hypoglycaemic agent against diabetes mellitus. Alx is a potent generator of reactive oxygen species ros, which can mediate betacell toxicity. Cortical sponge kidneys induced in rats by alloxan diabetes. Hepatoprotective and hypolipidemic effects of carthamus. Alloxaninduced diabetes is a model of type 1 dm, but type 2 dm is a more common risk factor for cvd. Mice administered a single intraperitoneal injection of alloxan monohydrate at a dose of 150 mgkg showed progression of typei diabetes symptoms figure 2. Diabetes was induced in group b and c by single intraperitoneal dose of alloxan 150mgkg body weight and rats having blood glucose 200mgdl were labeled as diabetic rats and included in study.
Research article alloxan induced diabetes mellitus in. After oral administration of each mesc singly or repeatedly to alloxan. Type 2 diabetes is a disorder of metabolism which usually affects insulin secretion and insulin uptake, with an increase in gluconeogenesis. An association between type 2 diabetes mellitus t2dm and nonalcoholic fatty liver disease nafld, which describes a wide spectrum of liver disorders from. Antidiabetic activity on extracts of mangifera indica in. Alloxaninduced diabetes, a common model for evaluating the. The cytotoxic action of both these diabetogenic agents is mediated by reactive oxygen species, however, the source of their generation is different in the case of alloxan and. Since then, alloxan diabetes has been commonly utilized as an animal model of insulindependent diabetes mellitus iddm.
Research article alloxan induced diabetes mellitus in wistar. It is a well known diabetogenic agent widely used to induce type 11 diabetes in animals viana et al. Alloxan is a urea derivative which causes selective necrosis of the. This article was published in diabetologia and it was entitled the mechanisms of alloxan and streptozotocininduced diabetes. Normal healthy mice 23 month old 2530 gm weight were used to develop diabetic mice by the injection of alloxan as described before. I dont know what is my mistake about inducing diabetes 1 by alloxan. It is interesting to note that this scientific subject has been dealt with, but one point in the article is the.
Type 1 diabetes results from irreversible damage of insulinproducing betacells. The effect of aepe leaf extract on blood glucose, insulin, hemoglobin and. This type of diabetes is caused by chronic pancreatitis or chronic drug therapy with glucocorticoids, thiazids diuretics, diazoxide, growth hormone and with some protease inhibitors e. Alloxan diabetes definition of alloxan diabetes by. Because it selectively kills the insulinproducing betacells found in the pancreas, alloxan is used to induce diabetes in laboratory animals. The current study investigated the effects of this compound referred to as compound k16 on diabetes using an alloxan. May 24, 2014 mice administered a single intraperitoneal injection of alloxan monohydrate at a dose of 150 mgkg showed progression of type i diabetes symptoms figure 2. Abstract as much as 20% of the women in menopause are reported to develop type i diabetes mellitus. Alloxaninduced diabetes is one of the widely used model to induce type i diabetes mellitus in the the experimental animals. Diabetes was induced in albino rats by administration of a single dose of alloxan monohydrate 5% 125 mgkg, i. Comment on anion gap toxicity in alloxan induced type 2. Alloxan and streptozotocin are widely used to induce experimental diabetes in animals.
The mechanisms of alloxan and streptozotocininduced diabetes. Obika4 abstract the purpose of this study was to determine the effects of diabetes mellitus and insulin resistance on semen parameters. Reninangiotensin system in phlorhizin compared with alloxan. According to eliakimikechukwu and obri 2009, alloxan selectively destroys. The control of hyperglycemia by estriol and progesterone in. Alloxan has been administered in single or multiple doses, through different routes intraperitoneal. Type 2 diabetes is by far the most common type of diabetes accounting for 85 to 95% of cases in developed nations and an even higher percentage in developing nations. In laboratory animals, diabetes can be induced with alloxan alx, a 2,4,5,6tetraoxopyrimidine. Studies of type 2 dm models should provide further understanding of the underlying mechanisms of diabetic atherosclerosis. Antihyperglycemic and antihyperlipidemic effects of aqueous. Before these animals were treated with alloxan, only those mice that had. It has been widely used to induce experimental diabetes in animals such as rabbits, rats, mice and dogs with different grades of disease severity by varying the dose of alloxan used 8.
Antidiabetic activity of piper betle in alloxan induced type 1 diabetic model rats html full text. Some histological changes in the intestines of alloxan induced diabetic mellitus albino rats. The control of hyperglycemia by estriol and progesterone in alloxan induced type i diabetes mellitus mice model through hepatic insulin synthesis. Diabetes was induced by intravenous injection of alloxan into 12 hfasted aj mice, followed by subcutaneous sensitization with ovalbumin. Pdf effect of oral cinnamon on histological damage. Hyperglycaemia was induced in fasted wistar rats with alloxan 150 mgkg body weight. Hepatoprotective and hypolipidemic effects of carthamus tinctorius linn. These results suggest that electrolyzed reduced water can prevent apoptosis of pancreatic. Alloxan is the next most commonly used chemical for induction of diabetes mellitus. We studied whether polyunsaturated fatty acids pufas can protect rat insulinoma rin5f cells against alloxan. The author declares that he has no conflicts of interest.
What are the advantages of streptozotocin over alloxan in. The mechanism of alloxan and streptozotocin action in b cells of the rat pancreas t. In alloxan treated diabetic rats, plasma renin activity pra is decreased. A histopathological investigation vikas kumar 1, shashi ranjan1, vivek akhouri1, md. Alloxan exerts its diabetogenic action when it is administered parenterally. In conclusion, our results suggest that glycolytic metabolism and glucose uptake are altered in diabetic atherosclerosis.
Protective effect of curcumin on quality parameters of sperm. The hypoglycemic effect of the kelp on diabetes mellitus. Pdf experimental diabetes induced by alloxan and streptozotocin. Type i diabetes was induced by giving single intravenous injection of alloxan monohydrate 70 mgkg body weight, dissolved in 0. The present study demonstrates that safflower seed oil might to be useful for the prevention of diabetes complications including hepatotoxicity and hyperlipidemia. After alloxan was injected and before kelp powder, forage was administered, and the animals showed typical signs of diabetes mellitus. Anion gap toxicity in alloxan induced type 2 diabetic rats treated with antidiabetic noncytotoxic bioactive compounds of ethanolic extract of moringa oleifera maxwell omabe,1,2 chibueze nwudele,1 kenneth nwobini omabe,3 and albert egwu okorocha3 1molecular cancer biology research group, molecular pathology and immunology division, department of medical laboratory sciences, school of biomedical. The control of hyperglycemia by estriol and progesterone. Protective effect of curcumin on quality parameters of. Alloxaninduced and insulinresistant diabetes mellitus.
Four groups n6 each received or not suspensions of cinnamon 50mg kg and. The mechanism of alloxan and streptozotocin action in b cells. Nov 10, 2010 blood glucose levels in alloxaninduced type 1 diabetes model mice were also significantly suppressed by feeding the mice with electrolyzed reduced water. The rats are divided into 6 groups and each group con tains 6 rats n 6 as follows. Others found a significant difference in alloxan plasma levels in children with and without diabetes type 1. After this procedure just 5 rats among 20 rats get diabetes 1 condition. Pdf the mechanism of alloxan and streptozotocin action. Streptozotocin stz is a widely used chemical for the induction of experimental diabetes in rodents. Alloxaninduced diabetes, a common model for evaluating. In 1838, wohler and liebig 1 synthesised a pyrimidine derivative, which they later called alloxan 2, 3. The mechanism of alloxan and streptozotocin action in b. Cytological and biochemical studies during the progression of alloxaninduced diabetes and possible protection of an aqueous leaf extract of costus afer.
Alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter. Dec 18, 2007 alloxan has two distinct pathological effects. Anion gap toxicity in alloxan induced type 2 diabetic rats. Here, we investigated the influence of diabetes on allergic airway inflammation in a model of experimental asthma in mice. In alloxantreated diabetic rats, plasma renin activity pra is decreased. Induction of experimental diabetes and treatment with aqueous suspension of ocimum sanctum. Effects of diabetes mellitus induced by alloxan on the. Alloxan is a urea derivative which causes selective necrosis of the pancreatic islet. Extracts were administered daily for 7d at doses of 200 and 400mgkg, p. Diabetes follows the alloxaninduced destruction of the pancreatic islet beta cell and, in the appropriate dose, it is selective to islet. Alloxaninduced and insulinresistant diabetes mellitus affect semen parameters and impair spermatogenesis in male rats a.
Suppressive effects of electrolyzed reduced water on alloxan. Alloxan induced diabetes is a model of type 1 dm, but type 2 dm is a more common risk factor for cvd. Altered glucose metabolism and hypoxic response in alloxan. Effects of three different doses of shilajit 50, 100 and. Mongolian oak cups, ethanol crude extract ece, ellagic acid and kaempferolderivatives, alloxaninduced rats, type 1 diabetes. In vitro study was conducted using rin5f cells while in vivo study was performed in wistar rats. Alloxan toxin causes diabetes suzy cohen suggests ways. The alloxan model of diabetes was first described in rabbits by dunn, sheehan and mcletchie in 1943. Alloxan toxin causes diabetes suzy cohen suggests ways to.
Diabetes mellitus is a metabolic disorder characterized by disturbances in. Phytochemical screening, antihyperglycemic and antihyperlipidemic effects of aqueous extracts of l. Average body weights reduced significantly before alloxan was injected f. The animals are markedly hyperglycemic, glycosuric, and ketoacidotic. The name is derived from allantoin, a product of uric acid excreted by the fetus into the allantois, and oxaluric acid derived from oxalic acid and urea, found in urine. The animals were checked for blood glucose level 48 h after alloxan injection, and. The mechanism of their action in b cells of the pancreas has been intensively investigated and now is quite well understood. Neurobehavioural and cognitive impairments are reportedly associated with both types of diabetes mellitus. This article is from international journal of biomedical science.
As seen from the data in figure 2, fasting blood glucose levels were reduced versus vehicle treatment following two weeks of oral treatment with glyburide 39. Diabetes was induced in 24 male albino rats using alloxan 120mgkg intraperitoneal. Safflower seed oil was investigated in diabetic rats. Cytological and biochemical studies during the progression of alloxan induced diabetes and possible protection of an aqueous leaf extract of costus afer. Alloxaninduced diabetes delays repair in a rat model of. Pdf the mechanism of alloxan and streptozotocin action in b. Alloxan induced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. However, the langerhans nucleus was partially damaged. Here, the authors show that human induced pluripotent stem cells from patients with type 1 diabetes can be differentiated into. Alloxan diabetes definition of alloxan diabetes by medical. Antidiabetic effect of ipomoea batatas in normal and alloxan. The responses are accompanied by corresponding inverse changes in. The seed oil of safflower at dose of 200 mgkg was administered as single dose per day to diabetic rats for a period of 28 days.
The researchers stated, these hydroxyl radicals are ultimately responsible for the death of the beta cells, which have a particularly. Diabetes follows the alloxan induced destruction of the pancreatic islet beta cell and, in the appropriate dose, it is selective to islet beta cell, producing minimal effects on other structures. Alloxan definition of alloxan by medical dictionary. No, type 2 dm is characterized by hyperglycemia due to decreased insulin release or insulin sensitivity due to partial damage of pancreatic beta cell, alloxan causes complete destruction of. Blood glucose lowering activity of aloe based composition. Pdf effect of oral cinnamon on histological damage caused. Effect of shilajit on blood glucose and lipid profile in. Department of biotechnology and genetic engineering 1, faculty of applied science. Antidiabetic activity of bauhinia forficata extracts in.
The researchers stated, these hydroxyl radicals are ultimately responsible for the death of the beta cells, which have a particularly low antioxidative defence capacity, and the. Antihyperglycemic and antihyperlipidemic effects of. Previous studies described that allergic diseases, including asthma, occur less often than expected in patients with type 1 diabetes. Antidiabetic effect of ipomoea batatas in normal and alloxaninduced diabetic rats. Effect of ocimum sanctum against alloxan induced diabetes. The dose of alloxan required for inducing diabetes depends on the animal. Alloxan is a cyclicurea derivative, which is a highly effective, diabetogenic agent used widely as a tool to induce experimental diabetes. This situation is somewhat analogous to type 1 diabetes in humans 27,28. Alloxan diabetes and streptozotocin diabetes figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected 22. In the first few days, there was necrosis of cells lining the distal portion of the proximal convoluted tubules, with focal involvement of distal convoluted tubules, and was especially marked at the corticomedullary junction. The mechanisms of alloxan and streptozotocininduced. The cessation of the ovarian syntheses of the female sex hormones is known to cause menopause in women, and the roles of estriol one of the most abundant estrogens and progesterone were investigated for hepatic. Some previous studies used higher stz dose variations to induce type 2 diabetes mellitus ghadge et al.
Antidiabetic effect of ipomoea batatas in normal and alloxan induced diabetic rats. Studies suggests that alloxan does not cause diabetes in humans. Beta cell mass restoration in alloxan diabetic mice treated with egf and gastrin. Effect of ocimum sanctum against alloxan induced diabetes and. In rat, mouse, rabbit, and dog, alloxaninduced diabetes is a model for type1 diabetes because a severe insulin deficiency is produced. This study aimed to investigate the potential histological repairing ability of damaged pancreatic tissue due to alloxaninduced diabetic rats. Diabetes was induced by administration of 120 mgkg alloxan monohydrate. Alloxan monohydrate is used to induce diabetes mellitus in albino rats in the dose of 120mgkg i. Alloxan induced and insulinresistant diabetes mellitus affect semen parameters and impair spermatogenesis in male rats 110 african journal of reproductive health african journal of reproductive health vol. In 2008, scientists closely examined how alloxan caused diabetes in animals.
Alloxan, on the other hand, as a model compound of ros mediated beta cell toxicity, is the agent with the greater impact upon the understanding of ros mediated mechanisms of beta cell death in type 1 and type 2 diabetes mellitus. Alloxaninduced diabetes mellitus how is alloxaninduced. Hepatoprotective and hypolipidemic effects of carthamus tinctorius oil in alloxan induced type 1 diabetic rats. In 1943, alloxan became of interest in diabetes research when dunn and mcletchie reported that it could induce diabetes in animals 4 as a result of the specific necrosis of the pancreatic beta cells 57. The metabolic disturbances were corrected after the leaf extracts were administered for 2 weeks, as shown by the normalisation of fasting blood glucose levels, glycolated haemoglobin, glycogen content and. However, the initial lesions on essential betacell structures are. Both effects collectively result in a pathophysiological state of insulindependent diabetes or type 1like diabetes mellitus in cells 78, 84. Alloxaninduced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. Streptozotocininduced type 1 diabetes in rodents as a. May 10, 2016 here, the authors show that human induced pluripotent stem cells from patients with type 1 diabetes can be differentiated into.
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